Genetic transformation of Drosophila with transposable element vectors.

by: GM Rubin, AC Spradling

Science (New York, N.Y.), Vol. 218, No. 4570. (22 October 1982), pp. 348-353.

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p-element, transformation, transposon

Abstract

Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.

@article{citeulike:3150643, abstract = {Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.}, author = {Rubin, G. M. and Spradling, A. C. }, citeulike-article-id = {3150643}, issn = {0036-8075}, journal = {Science (New York, N.Y.)}, keywords = {p-element, transformation}, month = {October}, number = {4570}, pages = {348--353}, posted-at = {2008-08-23 11:32:54}, priority = {2}, title = {Genetic transformation of Drosophila with transposable element vectors.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6289436}, volume = {218}, year = {1982} }

RIS record

TY - JOUR ID - citeulike:3150643 L3 - citeulike-article-id:3150643 N2 - Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes. IS - 4570 JF - Science (New York, N.Y.) SN - 0036-8075 EP - 353 TI - Genetic transformation of Drosophila with transposable element vectors. VL - 218 SP - 348 KW - p-element KW - transformation AU - Rubin, GM AU - Spradling, AC PY - 1982/10/22/ UR - http://view.ncbi.nlm.nih.gov/pubmed/6289436 ER -

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